Nanopore Sequencing

Introduction

As a next-generation sequencing technology, Nanopore has been widely used in scientific research. Biomarker has been committed to the exploration and development of the ONT platform. It currently has three generations of sequencing platforms such as MinION, GridION X5 and PromethION.

The ONT sequencing platform recognizes the base sequence by electrical signals. When the DNA/RNA single chain passes through the pore, different bases form a characteristic ion current change signal. By detecting these signals, the base sequence is obtained and sequencing is completed.


 
Nanopore MinION

miniION


MinION is the only portable real-time device for DNA and RNA sequencing.
Nanopore GridION

Grid ION x5


GridION X5 is a compact benchtop system designed to run and analyse up to five MinION Flow Cells.
grid

PromethION


PromethION offers the same real-time, long-read, direct DNA and RNA sequencing technology as MinION and GridION,and have 48 cells.

Product Type

 
Product The way of library constract Application direction
DNA Libraries sequence

Fragment screening

non-screening fragmentation 

MDA amplification

animals and plants genome (subsequent genome assembly)

structural variation analysis by individual sequencing (SV, CNV detection)

methylation analysis (5mC/6mA)

bacterial genome , fungal genome , microbial macrogenome
RNA-seq RNA-PCR; Direct-cDNA; Direct-RNA

full-length transcriptome, transcript quantification,

RNA base modification study,lncRNAs analysis.

 

Advantages and Application Areas

Since 2017 Biomarker has focused on the research and development of the Nanopore platform project。 It has completed the sequencing service of 150+ species, accumulated a wealth of project experience, and shortened the database sequencing period to 30 days(data200G).

1.The Long-reads generated by nanopore sequencing will be better applied to genome assembly;

2. Advantages of long reads for structural variation analysis; more conducive to the detection of SV, CNV;

3. Base modification detection; eg. Findding DNA methylation with high-throughput nanopore sequencing;

4. A full-length transcriptome is used analysis of species identification, unknow or fusion transcripts,and lncRNAs;

5。 Cost-effective and Real-time sequencing。

MinION due to its small size and easy operation can be directly used in clinical analysis, pathogen detection and other fields.

Partial Data Output

 
Species N50Len N90Len MeanLen MaxLen MeanQual
Flowers 26911 11976 21400 919516 8.24
Crop 45300 20534 31726 711760 8.38
Forest 31170 15045 23113 597729 8.8
Traditional Chinese medicine 42256 17856 29709 798932 8.49
Aquatic animal 36094 19304 28843 417624 8.33
Animal 26497 13306 21351 544239 8.5
 
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